Journal: PLoS ONE
Article Title: Integration-Free iPS Cells Engineered Using Human Artificial Chromosome Vectors
doi: 10.1371/journal.pone.0025961
Figure Lengend Snippet: (A) Unsupervised hierarchical clustering of global gene expression profiles from ES cells, iPS cells generated by retroviral or iHAC vectors, and MEFs. (B) Scatter plots of microarray data comparing ES cell line B6ES to iHAC1-iPS 1-B, iHAC-free iPS F-B1 and retroviral iPS 20D17 cells. Highlighted marks indicate core pluripotent markers, Nanog, Oct4 and Sox2. Red lines indicate two-fold changes in expression between samples.
Article Snippet: RNA (500 ng) was labeled with Cy3 using the Quick Amp Labeling Kit (Agilent) according to the manufacturer’s instructions. cRNA was column-purified using the RNeasy Kit (Qiagen) and hybridized to the Agilent Whole Mouse Genome Oligo Microarray (G4122A).
Techniques: Expressing, Generated, Microarray